Dating progressing slowly

by | 17-May-2020 16:09

In the present study, we analyzed the effect of HLA-B*5101 on clinical outcome in Japanese hemophiliacs infected with HIV-1.

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For sequence analysis, blood specimens were collected in EDTA.

Plasma and peripheral blood mononuclear cells (PBMCs) were separated from heparinized whole blood.

Therefore, it was speculated that only HLA-B*51 would play an important role in the control of HIV-1 replication in HIV-1-infected Japanese donors.

We showed previously that 2 Pol peptides and 1 Gag peptide were HLA-B*5101-restricted immunodominant CTL epitopes (45).

HLA class I-peptide tetrameric complexes (tetramers) were synthesized as described previously (2).

Four HIV-1 specific epitopes (Pol283-8, Pol743-9, Gag327-8, and Rev71-11) (45) were used for the refolding of HLA-B*5101 molecules.

Our results revealed a role for Pol283-8-specific HLA-B*5101-restricted HIV-1-specific CTLs in the long-lasting (approximately 25 years) control of HIV-1 replication.

One hundred eight Japanese hemophiliacs who had been infected with HIV-1 before 1985, mostly around 1983, were recruited for the present study, which was approved by the ethics committees of Kumamoto University and the National Center for Global Health and Medicine.

HLA-B*5101 hemophiliacs exhibited significantly slow progression. The analysis of HLA-B*5101-restricted HIV-1-specific cytotoxic T-lymphocyte (CTL) responses to 4 HLA-B*-restricted epitopes in 10 antiretroviral-therapy (ART)-free HLA-B*5101 hemophiliacs whose HIV-1 replication had been controlled for approximately 25 years had HIV-1 possessing the wild-type Pol283-8 sequence or the Pol283-8V mutant, which does not critically affect T-cell recognition, whereas other HLA-B*5101 hemophiliacs had HIV-1 with escape mutations in this epitope.

Dating progressing slowly

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