Dating progressing slowly
In the present study, we analyzed the effect of HLA-B*5101 on clinical outcome in Japanese hemophiliacs infected with HIV-1.
For sequence analysis, blood specimens were collected in EDTA.
Plasma and peripheral blood mononuclear cells (PBMCs) were separated from heparinized whole blood.
Therefore, it was speculated that only HLA-B*51 would play an important role in the control of HIV-1 replication in HIV-1-infected Japanese donors.
We showed previously that 2 Pol peptides and 1 Gag peptide were HLA-B*5101-restricted immunodominant CTL epitopes (45).
HLA class I-peptide tetrameric complexes (tetramers) were synthesized as described previously (2).